Introduction: Monoclonal antibodies are the antibodies derived from a single B-cell clone and are specific for single epitope. In 1975, George Kohler and Ceasar Milstein developed a method for preparing monoclonal antibodies. They were produced by Hybridoma Technology. A hybrid cell called Hybridoma, is made by the fusion of normal activated antibody producing B-cell […]
Homologous Recombination
Introduction: Chromosomes are not passed on intact, some part of maternaland paternal chromosome is exchanged through Homologous Recombination. Three models have been put forward to explain this phenomenon but only Holliday Model has been the most accepted. Holliday Model of Homologous Recombination: It was proposed by Robin Holliday in 1964. This model describes recombination […]
Mutations
Introduction: Mutation is any sudden hereditary change in the genetic make up of an individual which can be caused by simple recombination of genes. Term Mutation was coined by “Hugo De Vries”. Molecular Basis of Mutation: Sometimes process of DNA replication show inaccuracy which may occur within the DNA due to some internal or external, […]
Monoclonal Antibodies
Monoclonal antibodies are the antibodies derived from a single B-cell clone and are specific for single epitope. In 1975, George Kohler and Ceasar Milstein developed a method for preparing monoclonal antibodies. They were produced by Hybridoma Technology. A hybrid cell called Hybridoma, is made by the fusion of normal activated antibody producing B-cell with a […]